CORO™ Real-Time RT-PCR SARS-CoV-2 kit is an in-vitro diagnostics (IVD) test kit under Provisional Authorisation issued by Singapore’s Health and Sciences Authority.
The kit utilizes both the RNA-dependent RNA polymerase (RdRp) and Spike (S) genes for screening and confirms SARS-CoV-2 positive diagnosis by detecting the Nucleocapsid (N) gene.
The RT-PCR Kit is intended for use by trained clinical laboratory personnel specifically instructed and trained in the techniques of RT-PCR and in vitro diagnostic procedures.
Target viral RNA is extracted from the sample and converted into cDNA via Reverse transcription.
PCR amplification of target genetic material and Internal Control DNA to increase the amount of sample.
Simultaneously, the PCR amplicons are detected by fluorescent dye labelled probes.